![]() The type of gel you choose therefore depends on the type of question you are asking. This can result from both the types of amino acid used to construct them, as well as the types of modifications attached to them.ĭifferent types of electrophoresis gels are used to provide different types of information. Different proteins also have different charges. Chemical modifications attached to the protein also affect its size. However, gel electrophoresis can also be used to separate out proteins.ĭifferent proteins have different sizes, mainly due to the number of amino acid building blocks in their structure. Thanks to TV shows like CSI, many people are familiar with the use of gel electrophoresis to separate macromolecules like DNA. When is gel electrophoresis used to separate proteins? To test for genes associated with a particular disease.įind out more in the article, Gel electrophoresis can be used to find genes associated with a disease.See the video clip: Using gel electrophoresis to check a PCR reaction To get a DNA fingerprint so that you can look for evolutionary relationships among organisms.To get a DNA fingerprint for paternity testing.To get a DNA fingerprint for forensic purposes.Gel electrophoresis can be used for a range of purposes, for example: So instead of being at four, it's gonna be at, like, 3.When is gel electrophoresis used to separate DNA fragments? Then what happens? So instead of adding 1000 were subtracting 500 s in this case, we're still gonna get R two and R five pieces, so we'll just go ahead and write those than the four were, you know, subtracting 500 from. So 500 base pairs between the two restriction sites were deleted. So we're gonna put two bands at the five mark, And so what it would look like to the technician is just the two band showing up and then last but not least, we're adding 500 base pairs. What would the banding look like now? We're assuming that both of these cuts are going to occur still, So we still have our to K V and R five K be paid pieces that we need to add 1000 to the four key piece, but that's gonna make it five as well. And this one says, if 1000 base pairs of DNA were inserted between the two restriction sites, So now we're adding 1000 to this one. The total length then is going to just be 11 and that that's it for that piece. So now we're not getting this DNA cut at all. Let's say there's a mutation in both the first and the second one. Okay, so this first piece ends up being six k b in length, and then we start the second piece, which is five. Now all of the banding look like Well, so there's a mutation there. We would expect to see one at 21 at four. So what will the gel look like? So, for part A, we're gonna get lines at the length of these pieces. And so part A says this piece of DNA is cut just in this normal expected distribution that we are given in the problem. We're using the restriction sites at with a two que be peace A for KB size piece killed this piece and a five. So we have a, uh, piece of DNA, which pants sell sort of put off to the side and vertically. So here we're looking at, um, genetic analysis and in particular, the electrophoresis. If 500 bp of DNA between the two restriction sites were deleted, how would the banding pattern on the gel differ from the one that you drew in part $a ?$Īlready. If 1000 bp of DNA were inserted between the two restriction sites, how would the banding pattern on the gel differ from the one that you drew in part a?Į. If mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA, how will the banding pattern on the gel differ from the one that you drew in part a?ĭ. If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that you drew in part $a$ ?Ĭ. ![]() Draw a picture of the bands that will appear on the gel.ī. This piece of DNA is cut by $E c o \mathrm$, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Restriction mapping of a linear piece of DNA reveals the following EcoRI restriction sites.Ī. ![]()
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